Journal: bioRxiv

Article Title: Spontaneous activity of striatal projection neurons supports maturation of striatal inputs to substantia nigra dopaminergic neurons

doi: 10.1101/2024.01.05.574371

Figure Lengend Snippet: A. Example traces of spontaneous APs, recorded by whole-cell patch-clamp from striosomal dSPN of P4 D1-Cre/Ai14 animal and iSPN of P7 A 2a -Cre/Ai14 animal. B. Percent’s of spontaneously active (with AP firing) and silent (without AP firing) dSPNs and iSPNs in striosomes and matrix of P4-P14 animals. *p<0.05, **p<0.01, Chi-square test. Number of cells is indicated on the bars. Numbers of animals used are: dSPN P4 N=6, P7 N=9, P10 N=4; iSPN P7 N=4, P10 N=5, P14 N=3. Frequency of spontaneous APs in striosomal and matrix dSPNs of P4 animals and striosomal and matrix iSPNs of P7 animals from the same data set as the previous graph. C,D Spontaneous Ca 2+ oscillations in striosomal and matrix dSPNs (C) and iSPNs (D), recorded in striatal slices of P4 D1-Cre/Ai95D and P7 A 2a -Cre/Ai95D animals. Striosomal compartment border was marked on the maximal intensity projection of the movie, and ROIs corresponding to the active cells were selected on the standard deviation projection of the movie. Example traces show spontaneous Ca 2+ oscillations in striosomal and matrix cells of D1-Cre (C) and A 2a -Cre (D) slices. Percent’s of spontaneously active cells in striosomes and matrix from active cells in 8 KCl conditions are presented as single values per slice and mean±SEM. dSPNs: 17 slices for striosomes, 17 slices for matrix, 3 animals. iSPNs: 20 slices for striosomes, 20 slices for matrix, 5 animals. *p<0.05, **p<0.01, ***p<0.001, unpaired t-test.

Article Snippet: Whole-cell patch-clamp borosilicate-glass pipets (Harvard Apparatus) were obtained with a horizontal two-stage puller (P-1000, Sutter Instruments) and had a resistance typically between 4 and 6 MΩ.

Techniques: Patch Clamp, Standard Deviation

Journal: bioRxiv

Article Title: Spontaneous activity of striatal projection neurons supports maturation of striatal inputs to substantia nigra dopaminergic neurons

doi: 10.1101/2024.01.05.574371

Figure Lengend Snippet: Oprm1-Cre animals and wild-type littermates received cranial injection of AAV-hSyn-DIO-hM 4 D(G i ) into dorsal striatum at P1. At P6-P14 animals received s.c. injections of CNO twice a day. Adult animals were used for whole-cell patch clamp experiments, where DA neurons in SNpc were filled by biocytin for the analysis of the dendritic tree. A. Example images of DA cells filled by biocytin from SNpc of adult wild-type and Oprm1-Cre animals after viral transfection and neonatal injections of CNO. B. Sholl analysis graph showing the number of intersections of the dendrites with different radiuses, starting from the soma. Sample size, cells/animals: WT CNO 11/8, Oprm1-Cre CNO 7/5, WT saline 5/3, Oprm1-Cre saline 9/6. Data are presented as mean ± SEM.

Article Snippet: Whole-cell patch-clamp borosilicate-glass pipets (Harvard Apparatus) were obtained with a horizontal two-stage puller (P-1000, Sutter Instruments) and had a resistance typically between 4 and 6 MΩ.

Techniques: Injection, Patch Clamp, Transfection, Saline

Journal: Neural Regeneration Research

Article Title: Neuroprotective effects of ginsenoside Rg1-induced neural stem cell transplantation on hypoxic-ischemic encephalopathy

doi: 10.4103/1673-5374.156971

Figure Lengend Snippet: Effect of ginsenoside Rg1 on membrane function of induced neural stem cells at 7 days after differentiation (whole-cell patch clamp recording). Cell activation was measured using the whole-cell patch clamp technique. Amplitudes in the 20 μM ginsenoside Rg1 group were notably greater than those in the control group.

Article Snippet: Membrane electrical properties and sodium channel functionality were analyzed by whole-cell patch clamp (Harvard Apparatus, Cambridge, USA) on neuron-like cells 7 days after differentiation.

Techniques: Patch Clamp, Activation Assay